doi.bio/neb.ai

ai data

New England Biolabs: A Leading Provider of Synthetic Biology Tools

Overview

New England Biolabs (NEB) is an American life sciences company, founded in 1974 by Harvard Medical School professor, Donald "Don" Comb. The company is a world leader in the production and supply of reagents for the life science industry, with a specific focus on supporting synthetic biology and next-generation sequencing.

History

NEB was established as a cooperative laboratory of experienced scientists, initially producing restriction enzymes on a commercial scale. Over the years, NEB has expanded its offerings to include a range of products and services that support genome editing, synthetic biology, and next-generation sequencing. The company has also developed several free research tools, including REBASE, InBASE, and Polbase.

Products and Services

NEB offers a wide range of products and services for synthetic biologists, including:

Facilities and Distribution

NEB's headquarters are located in Ipswich, Massachusetts, USA, with eight subsidiary offices worldwide, including in Singapore, Canada, China, France, Germany, Japan, the UK, and Australia. The company also has distributors in South America, Australia, and other countries in Europe and Asia.

Social Responsibility and Sustainability

NEB is committed to environmental stewardship and has received ISO 14001 certification for its environmental management systems. The company actively promotes ecologically sound practices and strives for sustainability in its operations to protect natural resources.

Awards and Recognition

NEB has received recognition for its contributions to science, including the 2024 Passion in Science Awards, which honor those within the scientific community making a difference through mentorship, humanitarian efforts, environmental stewardship, and the arts.

Customer Support

NEB is dedicated to providing excellent customer support. As company policy, all scientists and some executives must work at least one day per month on the customer support telephone line, answering technical questions about the company's products.

Conclusion

New England Biolabs is a trusted and innovative provider of synthetic biology tools, with a rich history and a commitment to scientific advancement and sustainability. The company offers a comprehensive range of products and services that cater to the needs of synthetic biologists and life science researchers worldwide.

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The company has also developed several free research tools, including REBASE, InBASE, and Polbase.\n\n## Products and Services\nNEB offers a wide range of products and services for synthetic biologists, including:\n- Enzymes: NEB produces 230 recombinant and 30 native restriction enzymes, as well as nicking enzymes and DNA methylases.\n- Cloning and Synthetic Biology: The NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix enable one-step cloning and multiple DNA fragment assembly. \n- Nucleic Acid Purification: NEB provides purification kits for both DNA and RNA, including the Monarch Genomic DNA Purification Kit, which minimizes RNA contamination and allows for high-yield purification of large DNA fragments. \n- Library Prep Kits: NEB offers a range of library prep kits, such as the NEBNext Ultra II FS DNA library prep kit for next-generation sequencing and the NEBNext UltraExpress RNA Library Prep Kit for creating high-quality directional RNA libraries.\n- Genome Editing: In 2020, NEB signed an agreement granting the company rights to sell CRISPR/Cas9 tools and reagents for gene editing.\n- Research Tools: NEB provides free access to several research tools, including REBASE, a restriction enzyme database; Polbase, a polymerase database; and InBase, an intein database.\n\n## Facilities and Distribution\nNEB\'s headquarters are located in Ipswich, Massachusetts, USA, with eight subsidiary offices worldwide, including in Singapore, Canada, China, France, Germany, Japan, the UK, and Australia. The company also has distributors in South America, Australia, and other countries in Europe and Asia. \n\n## Social Responsibility and Sustainability\nNEB is committed to environmental stewardship and has received ISO 14001 certification for its environmental management systems. The company actively promotes ecologically sound practices and strives for sustainability in its operations to protect natural resources. \n\n## Awards and Recognition\nNEB has received recognition for its contributions to science, including the 2024 Passion in Science Awards, which honor those within the scientific community making a difference through mentorship, humanitarian efforts, environmental stewardship, and the arts. \n\n## Customer Support\nNEB is dedicated to providing excellent customer support. As company policy, all scientists and some executives must work at least one day per month on the customer support telephone line, answering technical questions about the company\'s products. \n\n## Conclusion\nNew England Biolabs is a trusted and innovative provider of synthetic biology tools, with a rich history and a commitment to scientific advancement and sustainability. 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arts.', documentids=['web-search0']), ChatCitation(start=3065, end=3175, text='all scientists and some executives must work at least one day per month on the customer support telephone line', documentids=['web-search2', 'web-search3']), ChatCitation(start=3187, end=3236, text="technical questions about the company's products.", documentids=['web-search2', 'web-search3'])], documents=[{'id': 'web-search0', 'snippet': "Announcing the 2024 Passion In Science Awards®\n\nNew England Biolabs is pleased to announce our 2024 Passion In Science Awards, recognizing those within the scientific community working to make a difference in the world through scientific mentorship, humanitarian efforts, environmental stewardship, or arts and creativity. Learn More\n\nFind the Support You Need\n\nBrowse our selection of literature and request your copy of the NEB Catalog and Technical Reference. View or Request Literature\n\nExplore our selection of helpful online tools for your research and experimental design. Browse All Tools\n\nVisit our video library to find tutorials, protocols and other informative content. View All Videos\n\nSearch from over 1,000 NEB protocols within our database.\n\nFind the Product You Need\n\nBrowse our products or use the Quick Order feature for fast ordering. Don't forget to visit our New Products and Special Offers pages to find out what's new at NEB.\n\nThe foundation for your innovation\n\nFind out how NEB can support your diagnostic and therapeutic manufacturing.\n\nRNase 4 is a single-stranded RNA endonuclease that cleaves RNA at uridine-purine sequences. It offers targeted cleavage and tolerates various uridine modifications. Compared to other RNases, it produces more heterogenous 3' end oligonucleotides. Learn More\n\nMonarch® Mag Viral DNA/RNA Extraction Kit\n\nThis kit enables reliable, high-throughput viral nucleic acid purification using a magnetic bead-based protocol. It has been tested for saliva and respiratory swab sample types and is compatible with wastewater samples after enrichment steps (not supplied). Learn More\n\nNEBNext UltraExpress™ RNA Library Prep Kit\n\nThe NEBNext UltraExpress™ RNA Library Prep Kit has a 3-hour library prep protocol & creates high-quality directional RNA libraries in a single day when paired with poly(A) mRNA enrichment or rRNA depletion kits. The kit features a single protocol, master mixed reagents, reduced incubation times, & fewer cleanup steps. Learn More\n\nReview NEB’s Latest Research\n\nRead about how NEB scientists use Golden Gate Assembly, data-optimized assembly design (DAD) and online tools to design one-pot assemblies of medium to high complexity genomes.\n\nThe NEBaseChanger® Primer Design Tool Tutorial video will explain how to design primers for site-directed and multi-site mutagenesis using NEBaseChanger. This tool is ideal for NEB Q5® and NEBuilder® HiFi DNA Assembly applications.\n\nLearn about an accessible method for shuffling DNA using NEBridge® Golden Gate Assembly to recombine proteins and find new properties.\n\nAn in-vitro strategy developed by NEB scientists expands cytosine deaminase applications and uncovers novel deaminases. They introduce a non-destructive 5-methylcytosine sequencing method (SEM-seq) utilizing a newly identified DNA deaminase.\n\nThe latest version of NEBaseChanger® is available to help design site-directed mutagenesis primers. Compatible with ambiguous bases, substitute based on amino acid position, batch upload for multiple primer sets and add mutations to primer tails.\n\nIn this issue, learn more about NEB’s history and how we are celebrating our 50th anniversary with customers. Learn more about our NEBNext UltraExpress™ library prep kits, Monarch® nucleic acid purification kits, and how we support therapeutic and diagnostic innovation.\n\nLearn About NEB's Research\n\nInterview with Jo Handelsman: Microbial Communities and Expanding Diversity in Science\n\nInterview with Jo Handelsman: Microbial Communities and Expanding Diversity in Science\n\nDr. Jo Handelsman, Director of the Wisconsin Institute for Discovery shares her expertise in microbiome research and the importance of community.\n\nListen to the Podcast\n\nOverview of the Q5® Site-Directed Mutagenesis Kit\n\nOverview of the Q5® Site-Directed Mutagenesis Kit\n\nLearn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit.\n\nCloser to the clinic: Optimized DNA assembly for personalized medicine research\n\nCloser to the clinic: Optimized DNA assembly for personalized medicine research\n\nIn this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine.\n\nOptimizing mRNA Capping and Analysis\n\nPosted on Monday, May 13, 2024 By Nicole Kelesoglu\n\nHave you heard about the higher activity of our new capping enzyme and our improved approach for LC-MS RNA cap analysis?\n\nPioneering High-Altitude Reforestation with Reforest the Tropics and New England Biolabs®\n\nPosted on Sunday, April 21, 2024 By Joanne Gibson, Ph.D.\n\nRead our blog post on the latest collaboration between NEB® and Reforest the Tropics in Costa Rica. This project is pushing the boundaries of reforestation, implementing innovative high-altitude strategies to enhance carbon sequestration and biodiversity. Join us in learning about this crucial step towards a sustainable future.\n\nPosted on Monday, March 25, 2024 By Nicole Kelesoglu\n\nStrategies for mRNA IVT templates, synthesis, capping and tailing, and purification that can streamline scale up or scale out for regulated markets.\n\nNEB 50-stop World Tour\n\nCombined VAAM & DGHM\n\nWuerzburg, Germany | June 2-5\n\nSalivaDirect Conference\n\nWashington, DC | June 5-7\n\nLondon NGS Symposium\n\nIf you don't see your country above, please visit our international site\n\nYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.\n\nYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.\n\nSign in to your NEB account\n\nTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.\n\nSign In Sign In Continue as Guest", 'timestamp': '2024-06-05T18:43:08', 'title': 'Reagents For the Life Sciences Industry | NEB', 'url': 'https://www.neb.com/en/'}, {'id': 'web-search1', 'snippet': "Sign In or Sign UpMy NEB\n\nFeatured Video The Philosophy Behind Our Science. 3 min 12 sec.\n\nFeatured Promotion How many Golden Butterflies have been found? Check the count here. Valid until July 15th, 2024.\n\nNEB Cohesive Ends, Issue II, 2024 In this issue, learn more about NEB’s history and how we are celebrating our 50th anniversary with customers and more.\n\nNew Product Monarch Mag Viral DNA/RNA Extraction Kit.\n\nDNA Modifying Enzymes and Cloning Technologies\n\nDNA Plasmids & Substrates\n\nNGS Sample Prep & Target Enrichment\n\nNucleic Acid Purification\n\nPCR, Polymerases & Amplification Technologies\n\nProtein Expression & Purification Technologies\n\nRestriction Endonucleases\n\nAll Product Categories\n\nSamples & Special Offers\n\nNew Lab Discount Create a Quote\n\nAlexa Fluor Conjugated Antibodies\n\nAntibody Sampler Kits\n\nCa, cAMP & Lipid Signaling\n\nCell Cycle / Checkpoint Control\n\nChromatin Regulation / Acetylation\n\nCytoskeletal Signaling\n\nFastScan™ Sandwich ELISA Kits\n\nGeneral Phospho-Ser/Thr/Tyr Antibodies\n\nGlucose / Energy Metabolism\n\nGrowth factors/cytokines\n\nImmunohistochemistry\n\nLymphocyte Signaling\n\nMAP Kinase Signaling\n\nNuclear Receptor Signaling\n\nPathScan Sandwich ELISA Kits\n\nPI3K / Akt Signaling\n\nRabbit Monoclonal Antibodies\n\nScreening Technologies\n\nSignalStar™ Multiplex IHC\n\nTGF-beta/Smad Signaling\n\nTranscription Factors\n\nTranslational Research\n\nTyrosine Kinases / Adaptors\n\nWnt / Hedgehog / Notch\n\nSignalStar™ Multiplex IHC Kits & Reagents\n\nView All Products Samples & Special Offers\n\nNew Lab Discount Create a Quote\n\nBurnaby, British Columbia\n\nHalifax, Nova Scotia\n\nSaskatoon, Saskatchewan\n\nVancouver, British Columbia\n\nVictoria, British Columbia\n\nRestriction Enzyme Tools\n\nCatalog & Literature Request\n\nTroubleshooting Guides\n\nUsage Guidelines/Tips\n\nShipping Information\n\nAmbient Temperature Shipping\n\nSamples & Special Offers\n\nNew Lab Discount Create a Quote\n\nCelebrating 50 years of passion for science.\n\nNEB was created by scientists for scientists, and we prioritize the advancement of science, stewardship of the environment, and giving back to the world around us. As we reflect on the last 50 years and look toward the future, we are excited to support your research and help you address the complex challenges facing the world today. We hope that by working together, we can shape the science of tomorrow. Learn How You Can Celebrate With Us\n\nStreamlined for speed.\n\nNEBNext UltraExpress™ DNA and RNA Library Prep Kits Learn More\n\nNEB Catalog & Technical Reference 2023/24\n\nThe NEB Catalog and Technical Reference provides information on new products and up-to-date technical charts, protocols and troubleshooting tips to aid experimental design. Request Your Free Copy Today\n\nFeatured Products & Offers\n\nLuna® Universal qPCR Master Mix & LunaScript RT SuperMix Kit\n\nExperience Best-In-Class Performance\n\nLuna® Universal qPCR Master Mix & LunaScript RT SuperMix Kit\n\nExperience Best-In-Class Performance\n\nExpires June 30th, 2024\n\nOneTaq®, Taq DNA Polymerase & Master Mixes\n\nOneTaq®, Taq DNA Polymerase & Master Mixes\n\nExpires June 30th, 2024\n\nCUT&Tag Companion Products and Validated Antibodies\n\nfrom Cell Signaling Technology®\n\nCUT&Tag Companion Products and Validated Antibodies\n\nfrom Cell Signaling Technology®\n\nExpires June 30th, 2024\n\nUnlock a World of Prizes!\n\nCelebrating 50 years of Passion for Science.\n\nFind a Golden Butterfly\n\nBeginning April 15th, find a golden butterfly on www.neb.com, on product samples, or at tradeshows and events.\n\nSpot a Golden Butterfly?\n\nClick or scan the QR code and complete the form to be eligible for a 50th anniversary prize pack.\n\nExpires July 15th, 2024.\n\nNEBuilder Assembly Tool\n\nDouble Digest Finder\n\nDNA Sequences and Maps Tool\n\nCompetitor Cross-Reference Tools\n\nNEB Golden Gate Assembly Tool\n\nRead Coverage Calculator\n\nNEBNext Custom RNA Depletion Design Tool\n\nEnGen sgRNA Template Oligo Designer\n\nNEB LAMP Primer Design Tool\n\nPCR Fidelity Estimator\n\nThermostable Ligase Reaction Temperature…\n\nNEBinspired designed to share inspirational stories about trends in the life sciences, lab tips to help you save time, and life lessons to reflect on.\n\nBrowse our science stories\n\nNEB researchers have authored or co-authored over 1,200 publications.\n\nAt NEB, basic research is a priority. To date, over 1,200 papers have been published by NEB scientists, in addition to many patents that have been received.\n\nLearn About NEB's Research\n\nFind the Support You Need\n\nBrowse our selection of literature and request your copy of the NEB Catalog and Technical Reference.\n\nView or Request Literature\n\nVisit our video library to find tutorials, protocols and other informative content.\n\nPassion in Science Awards\n\nBe inspired by customers who share NEB’s passion for scientific mentorship, environmental stewardship, humanities and the arts.\n\nLearn about the awards\n\nMolecular Cloning Series: Golden Gate Assembly 101\n\nMolecular Cloning Series: Golden Gate Assembly 101\n\nHear NEB Research Scientist Andy Sikkema, introduce Golden Gate Assembly and it’s uses, as well as tips for successful DNA assemblies.\n\nListen to the Podcast\n\nThe Effect of DNA Methylation on Restriction Digests\n\nThe Effect of DNA Methylation on Restriction Digests\n\nThis tutorial explains the effect of DNA methylation on restriction enzyme digestion; including dam, dcm and CpG methylation.\n\nCloser to the clinic: Optimized DNA assembly for personalized medicine research\n\nCloser to the clinic: Optimized DNA assembly for personalized medicine research\n\nIn this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine.\n\nListen to the Podcast\n\nDo you want to sign out ?", 'timestamp': '2024-06-05T04:37:00', 'title': 'New England Biolabs Canada | Reagents for the Life Science Industry', 'url': 'https://www.neb.ca/'}, {'id': 'web-search2', 'snippet': 'New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. NEB also provides free access to research tools such as REBASE, InBASE, and Polbase.\n\nThe company was founded in 1974 by Donald "Don" Comb, a Harvard Medical School professor, as a cooperative laboratory of experienced scientists and initially produced restriction enzymes on a commercial scale. Comb held the CEO title until 2005 when, at 78 years old, he moved from management back into research at the firm.\n\nNEB received approximately $1.7 million in Small Business Innovation Research (SBIR) grants between 2009 and 2013 for this research.\n\nNEB produces 230 recombinant and 30 native restriction enzymes for genomic research, as well as nicking enzymes and DNA methylases. It pursues research in areas related to proteomics, DNA Sequencing, and drug discovery. NEB scientists also conduct basic research in Molecular Biology and Parasitology.\n\nThe company has subsidiaries in Singapore, Canada, China, France, Germany, Japan, the U.K., and Australia, and distributors in South America, Australia, and other countries in Europe and Asia. Its headquarters are in Ipswich, MA. Development of the current headquarters began in 2000, and was completed in 2005. Donald Comb served as the company\'s Chairman and CEO from the company\'s founding in 1974, until 2005. In 2005, he was replaced as chief executive by James Ellard, though Comb continued to serve as Chairman of the Board of Directors. In October 2020 Comb passed away at the age of 93. NEB employs over 450 people at its headquarters. As company policy, all scientists and some executives must work at least one day per month on the customer support telephone line, answering technical support questions about the company\'s products. In 2022 Jim Ellard stepped down as CEO, but remained chairman of the board of directors, he was succeeded by Salvatore (Sal) Russello, previously NEB\'s director of OEM & customized solutions.\n\nSir Richard John Roberts is the company\'s Chief Scientific Officer. He shared the 1993 Nobel Prize in Physiology or Medicine with Phillip Allen Sharp for the discovery of introns in eukaryotic DNA and the mechanism of gene-splicing.\n\nIn 2015, NEB committed to establishing a GMP manufacturing facility near its headquarters in Ipswich, Massachusetts, and the 40,000-sq-ft facility was completed in 2018. The multi-product Rowley Cleanroom Manufacturing Facility makes GMP-grade products and has a 10,000-sq-ft mechanical mezzanine.\n\nApplications and Tools\n\nIn January 2017, NEB released Luna universal quantitative real-time polymerase chain reaction (qPCR) and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) kits. The Luna kits are used for DNA or RNA quantitation.\n\nIn December 2017, the company released the NEBNext Ultra II FS DNA library prep kit for next-generation sequencing (NGS). In October 2019, NEB released a new RNA depletion product, the NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) and NEBNext rRNA Depletion Kit (Bacteria). The kits offer specific depletion of the RNA species that interfere with the analysis of coding and non-coding RNAs. That same month, the company announced its NEBNext Direct Genotyping Solution. The product delivers a one day, automatable genotyping workflow for a variety of applications in Agricultural biotechnology.\n\nIn January 2020, NEB signed an agreement with ERS Genomics Limited that gave NEB rights to sell CRISPR/Cas9 tools and reagents, used for gene editing.\n\nCloning and synthetic biology\n\nThe NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix enable one-step cloning and multiple DNA fragment assembly. The proprietary DNA polymerase in the NEBuilder HiFi enzyme mix can assemble DNA fragments ranging from 100 bp to 19 kb. NEB also offers the Gibson Assembly Master Mix.\n\nMonarch nucleic acid purification\n\nNEB provides purification kits for both DNA and RNA. In May 2019, NEB released the Monarch Genomic DNA Purification Kit which is designed to minimize RNA contamination and allow high-yield purification of large DNA fragments. NEB’s nucleic acid purification products have been used in various studies, including:\n\nPurification of genomic DNA used to discover naturally-occurring DNA modifications in bacteriophages.\n\nPurification of RNA from wound biopsies to study the relationship between genetics, wound microbiome diversity, and wound healing.\n\nPurification of genomic DNA from squid embryos used in the first gene knockout in a cephalopod.\n\nPurification of RNA from mouse samples in a study identifying a pathway that selectively regulates cancer stem cells, which may be responsible for treatment resistance, tumor metastasis, and disease recurrence.\n\nPurification of total RNA from Arabidopsis seedlings in a study demonstrating the first known response by a biological receptor to radio frequency exposure.\n\nResponse to COVID-19\n\nNew England Biolabs developed a colorimetric loop-mediated isothermal amplification (LAMP) assay for research use. This assay can be used to test for the presence of virus through nucleic acid detection, returning results in only 30 minutes. In 2020, the LAMP method was one of several molecular tests used to detect RNA from SARS-CoV-2, a strain of coronavirus that causes COVID-19.\n\nRNA isolation kits were also used to develop assays to detect SARS-CoV-2. NEB’s Monarch Total RNA Miniprep Kit was not designed specifically for viral RNA extraction, but it was successfully used by different companies to extract viral RNA from biological samples. NEB also released a supplementary protocol for processing saliva, buccal swabs, and nasopharyngeal samples.\n\nThree next-generation sequencing kits to support SARS-CoV-2 monitoring were launched in February, 2021. These kits, based on ARTIC Network protocols, provide virus transmission and evolution insights.\n\nIn April, 2021, the Color SARS-CoV-2 RT-LAMP Diagnostic Assay, utilizing New England Biolabs reagents, was approved for emergency use at Color Health Inc in Burlingame, California.\n\nThe company runs free scientific databases. REBASE, the restriction enzyme database, contains the details of commercial and research endonucleases. In 2011 the company founded Polbase, an online database which provides information specifically about polymerases. Another free NEB database is InBase, an intein database, which includes the Intein Registry and information about each intein.\n\nIn 2001, NEB co-founded the marine DNA library Ocean Genome Legacy (OGL), which according to the Boston Globe, “catalogues samples of organisms from all over the world, to be made available to scientists for research”. Though originally located on the NEB campus, OGLF relocated to the Nahant campus of Northeastern University in 2014. To enable point-of-use sales of its reagents, NEB created a digital interface for enzyme-housing freezers to be used at customer storage sites, through a partnership with Ionia Corp. and Salesforce.com. The data is used by the company for both sales logistics and as a part of future enzyme research development. It has also partnered with Harvard University on recycling and reclamation initiatives when its products and packaging come to the end of their use or lifecycle. As of 2015, NEB also had a distribution agreement with VWR.\n\nIn June 2019, NEB, Waters, and Genos announced they would work together on The Human Glycome Project, a global initiative to map the structure and function of human glycans. NEB will supply a version of its Rapid PNGase F technology to aid in increased sample preparation and improve process throughput.\n\nThat same month, NEB entered a partnership with Bioz, Inc., an artificial intelligence technology company, to provide its customers with access to examples of real-world applications of its products.', 'timestamp': '2024-06-05T22:38:05', 'title': 'New England Biolabs - Wikipedia', 'url': 'https://en.wikipedia.org/wiki/NewEnglandBiolabs'}, {'id': 'web-search3', 'snippet': 'New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. NEB also provides free access to research tools such as REBASE, InBASE, and Polbase.\n\nThe company was founded in 1974 by Donald "Don" Comb, a Harvard Medical School professor, as a cooperative laboratory of experienced scientists and initially produced restriction enzymes on a commercial scale. Comb held the CEO title until 2005 when, at 78 years old, he moved from management back into research at the firm.\n\nNEB received approximately $1.7 million in Small Business Innovation Research (SBIR) grants between 2009 and 2013 for this research.\n\nNEB produces 230 recombinant and 30 native restriction enzymes for genomic research, as well as nicking enzymes and DNA methylases. It pursues research in areas related to proteomics, DNA Sequencing, and drug discovery. NEB scientists also conduct basic research in Molecular Biology and Parasitology.\n\nThe company has subsidiaries in Singapore, Canada, China, France, Germany, Japan, the U.K., and Australia, and distributors in South America, Australia, and other countries in Europe and Asia. Its headquarters are in Ipswich, MA. Development of the current headquarters began in 2000, and was completed in 2005. Donald Comb served as the company\'s Chairman and CEO from the company\'s founding in 1974, until 2005. In 2005, he was replaced as chief executive by James Ellard, though Comb continued to serve as Chairman of the Board of Directors. In October 2020 Comb passed away at the age of 93. NEB employs over 450 people at its headquarters. As company policy, all scientists and some executives must work at least one day per month on the customer support telephone line, answering technical support questions about the company\'s products. In 2022 Jim Ellard stepped down as CEO, but remained chairman of the board of directors, he was succeeded by Salvatore (Sal) Russello, previously NEB\'s director of OEM & customized solutions.\n\nSir Richard John Roberts is the company\'s Chief Scientific Officer. He shared the 1993 Nobel Prize in Physiology or Medicine with Phillip Allen Sharp for the discovery of introns in eukaryotic DNA and the mechanism of gene-splicing.\n\nIn 2015, NEB committed to establishing a GMP manufacturing facility near its headquarters in Ipswich, Massachusetts, and the 40,000-sq-ft facility was completed in 2018. The multi-product Rowley Cleanroom Manufacturing Facility makes GMP-grade products and has a 10,000-sq-ft mechanical mezzanine.\n\nApplications and Tools\n\nIn January 2017, NEB released Luna universal quantitative real-time polymerase chain reaction (qPCR) and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) kits. The Luna kits are used for DNA or RNA quantitation.\n\nIn December 2017, the company released the NEBNext Ultra II FS DNA library prep kit for next-generation sequencing (NGS). In October 2019, NEB released a new RNA depletion product, the NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) and NEBNext rRNA Depletion Kit (Bacteria). The kits offer specific depletion of the RNA species that interfere with the analysis of coding and non-coding RNAs. That same month, the company announced its NEBNext Direct Genotyping Solution. The product delivers a one day, automatable genotyping workflow for a variety of applications in Agricultural biotechnology.\n\nIn January 2020, NEB signed an agreement with ERS Genomics Limited that gave NEB rights to sell CRISPR/Cas9 tools and reagents, used for gene editing.\n\nCloning and synthetic biology\n\nThe NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix enable one-step cloning and multiple DNA fragment assembly. The proprietary DNA polymerase in the NEBuilder HiFi enzyme mix can assemble DNA fragments ranging from 100 bp to 19 kb. NEB also offers the Gibson Assembly Master Mix.\n\nMonarch nucleic acid purification\n\nNEB provides purification kits for both DNA and RNA. In May 2019, NEB released the Monarch Genomic DNA Purification Kit which is designed to minimize RNA contamination and allow high-yield purification of large DNA fragments. NEB’s nucleic acid purification products have been used in various studies, including:\n\nPurification of genomic DNA used to discover naturally-occurring DNA modifications in bacteriophages.\n\nPurification of RNA from wound biopsies to study the relationship between genetics, wound microbiome diversity, and wound healing.\n\nPurification of genomic DNA from squid embryos used in the first gene knockout in a cephalopod.\n\nPurification of RNA from mouse samples in a study identifying a pathway that selectively regulates cancer stem cells, which may be responsible for treatment resistance, tumor metastasis, and disease recurrence.\n\nPurification of total RNA from Arabidopsis seedlings in a study demonstrating the first known response by a biological receptor to radio frequency exposure.\n\nResponse to COVID-19\n\nNew England Biolabs developed a colorimetric loop-mediated isothermal amplification (LAMP) assay for research use. This assay can be used to test for the presence of virus through nucleic acid detection, returning results in only 30 minutes. In 2020, the LAMP method was one of several molecular tests used to detect RNA from SARS-CoV-2, a strain of coronavirus that causes COVID-19.\n\nRNA isolation kits were also used to develop assays to detect SARS-CoV-2. NEB’s Monarch Total RNA Miniprep Kit was not designed specifically for viral RNA extraction, but it was successfully used by different companies to extract viral RNA from biological samples. NEB also released a supplementary protocol for processing saliva, buccal swabs, and nasopharyngeal samples.\n\nThree next-generation sequencing kits to support SARS-CoV-2 monitoring were launched in February, 2021. These kits, based on ARTIC Network protocols, provide virus transmission and evolution insights.\n\nIn April, 2021, the Color SARS-CoV-2 RT-LAMP Diagnostic Assay, utilizing New England Biolabs reagents, was approved for emergency use at Color Health Inc in Burlingame, California.\n\nThe company runs free scientific databases. REBASE, the restriction enzyme database, contains the details of commercial and research endonucleases. In 2011 the company founded Polbase, an online database which provides information specifically about polymerases. Another free NEB database is InBase, an intein database, which includes the Intein Registry and information about each intein.\n\nIn 2001, NEB co-founded the marine DNA library Ocean Genome Legacy (OGL), which according to the Boston Globe, “catalogues samples of organisms from all over the world, to be made available to scientists for research”. Though originally located on the NEB campus, OGLF relocated to the Nahant campus of Northeastern University in 2014. To enable point-of-use sales of its reagents, NEB created a digital interface for enzyme-housing freezers to be used at customer storage sites, through a partnership with Ionia Corp. and Salesforce.com. The data is used by the company for both sales logistics and as a part of future enzyme research development. It has also partnered with Harvard University on recycling and reclamation initiatives when its products and packaging come to the end of their use or lifecycle. As of 2015, NEB also had a distribution agreement with VWR.\n\nIn June 2019, NEB, Waters, and Genos announced they would work together on The Human Glycome Project, a global initiative to map the structure and function of human glycans. NEB will supply a version of its Rapid PNGase F technology to aid in increased sample preparation and improve process throughput.\n\nThat same month, NEB entered a partnership with Bioz, Inc., an artificial intelligence technology company, to provide its customers with access to examples of real-world applications of its products.', 'timestamp': '2024-06-05T22:38:05', 'title': 'New England Biolabs - Wikipedia', 'url': 'https://en.wikipedia.org/wiki/NewEnglandBiolabs'}, {'id': 'web-search4', 'snippet': "NEB Overview Founded in the mid-1970s by scientists committed to developing innovative products for the life sciences, New England Biolabs is a recognized world leader in the discovery, development and commercialization of reagents for genomic research.\n\nIn loving memory – Donald G. Comb Don’s commitment to science led to the establishment of NEB in 1974, and his scientific vision shaped the company as the leader in the discovery and production of recombinant enzymes for molecular biology applications.\n\nNews and Press Releases Learn more about New England Biolabs by viewing our recent press releases and local news articles.\n\nBrowse press releases\n\nLeadership Meet the leadership team at New England Biolabs and learn more about their backgrounds.\n\nResearch at NEB Basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs.\n\nEnvironmental Commitment NEB continuously strives to promote ecologically sound practices and environmental sustainability in order to protect our natural resources, both locally and globally.\n\nSocial Responsibility & Sustainability Learn more about NEB’s commitment to environmental stewardship, scientific advancement and education, humanitarian efforts and employee well-being.\n\nISO Certifications NEB products are manufactured in our ISO 13485:2016 and ISO 9001:2015 certified facility in Ipswich, MA, USA. We have also received ISO 14001 certification, a quality standard for environmental management systems.\n\nView ISO certifications\n\nPassion in Science Awards Be inspired by customers who share NEB’s passion for scientific mentorship, environmental stewardship, humanities and the arts.\n\nLearn about the awards\n\nBusiness Dev. Opportunities The business development team at NEB operates on a global basis to establish and support new ventures, partnerships and technology acquisitions.\n\nCareers Employment at New England Biolabs (NEB) offers a challenging and creative work environment in a state-of-the-art research and production facility, with a team of exceptional scientists and professional staff.\n\nExplore career opportunities\n\nContact Us Use this form to contact NEB with any questions you may have regarding a product or current order, or to provide feedback on our products and services.\n\nIf you don't see your country above, please visit our international site\n\nYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.\n\nYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.\n\nSign in to your NEB account\n\nTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.\n\nSign In Sign In Continue as Guest", 'timestamp': '2024-03-22T19:12:12', 'title': '\u200bAbout NEB | NEB', 'url': 'https://www.neb.com/about-neb'}, {'id': 'web-search5', 'snippet': 'New England Biolabs Inc. Established in the mid-1970s as a co-operative laboratory of experienced scientists, New England Biolabs (NEB) is a world leader in the production and supply of reagents for the life science industry. NEB is headquartered in Ipswich. Massachusetts, USA and has eight subsidiary offices over the world.\n\nNew England Biolabs France SAS Created in 2011 and located on the Evry Genopole Campus (South of Paris), the French team is committed to giving you the best quality of service. The complete team (administrative, technical and commercial) is devoted to help you the best way in your research.\n\nBasic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs.\n\nIt is our goal to deliver best-in-class product quality and technical support. We are constantly improving the stringency and range of our quality controls.\n\nEnvironmental commitment\n\nNEB continuously strives to promote ecologically sound practices and environmental sustainability to ensure the protection of natural resources.\n\nA specialized team is at your service to answer any technical questions you may have related to our products.\n\nFind out how NEB can help streamline your product development providing solutions that allow you to access novel products and meet quality specifications.\n\nWith the NEB Freezer Program, you can enjoy convenient and affordable access to NEB’s high quality reagents, any time of the day.\n\nNEB has succeeded in offering us a set of RT-qPCR solutions perfectly suited to our needs for working urgently on the SARS-CoV-2 - and to deliver us in a few days in full containment. Thank you for the great products and services that make NEB worth. Congrats also for your eco-packaging.\n\nEquipe CEMIPAI, UMS3725CNRS, Montpellier\n\nI have been using NEB products since the beginning of my career with great satisfaction and it was therefore quite natural that I became loyal again when I returned to the bench after several years without any experimental activity. So of course I discovered new products and methods, and the use of tools like the NEBCloner, the NEBCalculator have become a very precious help on a daily basis. In addition, the contact with the sales representative in charge of our laboratory is really very satisfying and I am always delighted to interact with her.\n\nVirginie Molle, PhDLPHI, Montpellier\n\nResponsible for testing the feasibility of the protocol described for a mass screening of SarsCov2, I naturally contacted the technical support of New England Biolabs. Indeed, we needed to quickly identify the best reagents and experimental conditions to set up. Thanks to regular and almost weekly discussions, we were able to identify the best solutions suitable for this approach. Thanks to these exchanges and the contacts of this service with the R&D of New England Biolabs, I established a protocol which we were able to quickly test in the laboratory. The solutions identified allowed us to validate the approach from our first tests. I cannot thank Karine Trottier and Morgane Moreau enough for their availability and responsiveness during our discussions on this new multiplex RT-PCR protocol coupled with detection by NGS. I therefore strongly recommend that anyone wishing to call on NEB technical support not to hesitate, they will find scientists of great skill.\n\nFrancis RousseauNGS Scientific Expert, Biomnis\n\nAs the generation and analysis of high-throughput sequencing data are two of the main axes of our research team, we wanted to work with the company New England Biolabs. Our contacts, Mmes Moreau and Gourvennec, as well as Mr. Doll, were able to demonstrate professionalism and attentive listening. Always aware of our technical and biological constraints, they have been by our side at every stage, undeniably providing us with support and expertise.\n\nClaudia CaradecBiology Engineer, Strasbourg Univ./ UMR7156\n\nWe would like to warmly thank you for your outstanding help and kind words during the lockdown. Through your attitude, you helped us to get the required reagents to conduct our research in a timely manner. We have now shared our results on BioRxiv.\n\nÉtienne CoyaudPRISM INSERM U1192 - Université de Lille\n\nAvis à nos clients !\n\nFermeture exceptionnelle de nos locaux !\n\nExceptionnellement, compte tenu des deux jours fériés consécutifs mercredi et jeudi prochain (8 et 9 mai), aucune livraison ne pourra être assurée la semaine prochaine (du mardi 7 au vendredi 10 mai). Nous reprendrons nos livraisons le mardi 13 mai.\n\nNous serons également exceptionnellement fermés le vendredi 10 mai.\n\nVeuillez nous excuser pour ce désagrément.', 'timestamp': '2024-05-09T17:42:07', 'title': 'About NEB - New England Biolabs France', 'url': 'https://www.neb-online.fr/en/about-neb/'}], issearchrequired=None, searchqueries=[ChatSearchQuery(text='new england biolabs', generationid='7463dd46-783c-483a-bed9-83be230c4dfb'), ChatSearchQuery(text='new england biolabs history', generationid='7463dd46-783c-483a-bed9-83be230c4dfb')], searchresults=[ChatSearchResult(searchquery=ChatSearchQuery(text='new england biolabs', generationid='7463dd46-783c-483a-bed9-83be230c4dfb'), connector=ChatSearchResultConnector(id='web-search'), documentids=['web-search0', 'web-search1', 'web-search2'], errormessage=None, continueonfailure=None), ChatSearchResult(searchquery=ChatSearchQuery(text='new england biolabs history', generationid='7463dd46-783c-483a-bed9-83be230c4dfb'), connector=ChatSearchResultConnector(id='web-search'), documentids=['web-search3', 'web-search4', 'web-search5'], errormessage=None, continueonfailure=None)], finishreason='COMPLETE', toolcalls=None, chathistory=[MessageUser(message='Please write a wiki article about New England Biolabs tailored for synthetic biologists interested in buying equiment from them', toolcalls=None, role='USER'), MessageChatbot(message='# New England Biolabs: A Leading Provider of Synthetic Biology Tools\n\n## Overview\nNew England Biolabs (NEB) is an American life sciences company, founded in 1974 by Harvard Medical School professor, Donald "Don" Comb. The company is a world leader in the production and supply of reagents for the life science industry, with a specific focus on supporting synthetic biology and next-generation sequencing. \n\n## History\nNEB was established as a cooperative laboratory of experienced scientists, initially producing restriction enzymes on a commercial scale. Over the years, NEB has expanded its offerings to include a range of products and services that support genome editing, synthetic biology, and next-generation sequencing. The company has also developed several free research tools, including REBASE, InBASE, and Polbase.\n\n## Products and Services\nNEB offers a wide range of products and services for synthetic biologists, including:\n- Enzymes: NEB produces 230 recombinant and 30 native restriction enzymes, as well as nicking enzymes and DNA methylases.\n- Cloning and Synthetic Biology: The NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix enable one-step cloning and multiple DNA fragment assembly. \n- Nucleic Acid Purification: NEB provides purification kits for both DNA and RNA, including the Monarch Genomic DNA Purification Kit, which minimizes RNA contamination and allows for high-yield purification of large DNA fragments. \n- Library Prep Kits: NEB offers a range of library prep kits, such as the NEBNext Ultra II FS DNA library prep kit for next-generation sequencing and the NEBNext UltraExpress RNA Library Prep Kit for creating high-quality directional RNA libraries.\n- Genome Editing: In 2020, NEB signed an agreement granting the company rights to sell CRISPR/Cas9 tools and reagents for gene editing.\n- Research Tools: NEB provides free access to several research tools, including REBASE, a restriction enzyme database; Polbase, a polymerase database; and InBase, an intein database.\n\n## Facilities and Distribution\nNEB\'s headquarters are located in Ipswich, Massachusetts, USA, with eight subsidiary offices worldwide, including in Singapore, Canada, China, France, Germany, Japan, the UK, and Australia. The company also has distributors in South America, Australia, and other countries in Europe and Asia. \n\n## Social Responsibility and Sustainability\nNEB is committed to environmental stewardship and has received ISO 14001 certification for its environmental management systems. The company actively promotes ecologically sound practices and strives for sustainability in its operations to protect natural resources. \n\n## Awards and Recognition\nNEB has received recognition for its contributions to science, including the 2024 Passion in Science Awards, which honor those within the scientific community making a difference through mentorship, humanitarian efforts, environmental stewardship, and the arts. \n\n## Customer Support\nNEB is dedicated to providing excellent customer support. As company policy, all scientists and some executives must work at least one day per month on the customer support telephone line, answering technical questions about the company\'s products. \n\n## Conclusion\nNew England Biolabs is a trusted and innovative provider of synthetic biology tools, with a rich history and a commitment to scientific advancement and sustainability. The company offers a comprehensive range of products and services that cater to the needs of synthetic biologists and life science researchers worldwide.', toolcalls=None, role='CHATBOT')], prompt=None, meta=ApiMeta(apiversion=ApiMetaApiVersion(version='1', isdeprecated=None, isexperimental=None), billedunits=ApiMetaBilledUnits(inputtokens=8288, outputtokens=725, searchunits=None, classifications=None), tokens=ApiMetaTokens(inputtokens=9018, output_tokens=725), warnings=None))




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