Q5® High-Fidelity DNA Polymerase 1
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
Highest fidelity amplification (~280X higher than Taq)
Ultra-low error rates
Superior performance for a broad range of amplicons (from high AT to high GC)
Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and master mix formats (NEB #M0492, M0494)
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Catalog # Concentration Size
M0491S 2,000 units/ml 100 units
M0491L 2,000 units/ml 500 units
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
This product is related to the following categories: Q5® High-Fidelity DNA Polymerases Products
This product can be used in the following applications: Gibson Assembly®, Long Range PCR, Fast PCR, | More +