The RNeasy Plant Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The RNeasy Plant Mini Kit includes QIAshredder spin columns for homogenizing and filtering viscous plant or fungal lysates, and RNeasy spin columns for purifying up to 100 μg of high-quality RNA using silica-membrane technology. Purification can be fully automated on the QIAcube Connect. The kit can also be used in combination with a TissueRuptor or TissueLyser system, which provide efficient disruption and homogenization of plant samples.
The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10–100 mg tissue, or 100–1 x 107 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). The binding capacity of the spin-columns is up to 100 µg RNA. The typical yield from 100 mg plant tissue is between 25 µg and 65 µg of RNA, although amounts of RNA can vary due to the developmental stage and growth conditions of the samples (see table "Yield from 100 mg tissue").
Plants Filamentous fungi Anemone sp. Arabidopsis thaliana1 Begonia sp. Beta vulgaris (sugar beet)2 Chlamydomonas reinhardii (unicellular alga) Chrysanthemum Clarkia spp.3 Daucus carota (carrot)4 Diascia sp. Dendranthema sp. Euglena gracilis (unicellular alga) Funaria hygrometrica (moss) Hordeum vulgare (barley) Lycopersicon esculentum (tomato)5 Malus sp. (apple)6 Nicotiana tabacum (tobacco) Oryza sativa (rice) Pelargonium sp. (geranium) Petroselinum crispum (parsley)7 Pisum sativum (pea) Prunus sp. (cherry)6 Ranunculus sp. Ribes nigrum (black currant) Riccia fluitans (liverwort) Sinapis arvensis (mustard) Solanum tuberosum (potato)8 Spinacia oleracea (spinach) Surfinia sp. Triticum aestivum (wheat) Vitis sp. (grapevine)6 Zea mays (maize) Acremonium crysogenum9 Fusarium avenaceum9 Young leaves or needles, except as indicated:1 Flowers, buds, siliques, roots; 2 roots; 3 petals, stamen, stigma, sepals, style; 4 cultured cells; 5 mature flower, expanded leaves, shoot tip, stem, green fruit; 6 budwood; 7 seedlings; 8 dormant tuber; 9 mycelium
Sample type Yield Arabidopsis leaves 35 µg Maize leaves 25 µg Tomato leaves 65 µg Tobacco leaves 60 µg The purified plant RNA, eluted in 30–100 µl volume, includes viral RNA (see figure " Detection of viruses"). All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications (see figure " Tissue specificity of histone H4 expression").
The RNeasy Plant Mini Kit provides QIAshredder columns for homogenization and filtration of viscous plant or fungal lysates by microcentrifugation in combination with RNeasy Mini spin columns for RNA purification. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using convenient on-column DNase treatment during the RNeasy procedure.
Samples are first lysed and then homogenized in the QIAshredder columns. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane. RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in water (see flowchart " RNeasy Plant Mini procedure").
RNA purified with RNeasy technology is high-quality and is ideal for use in all applications. Downstream applications include:
Northern, dot, and slot blotting End-point RT-PCR Quantitative, real-time RT-PCR Array analysis Poly A+ RNA selection Supporting data and figures RNeasy Plant Mini procedure. RNeasy Plant Mini procedure. Specifications Features Specifications Applications PCR, qPCR, real-time RT-PCR, microarray Elution volume 30–100 µl Sample amount 10–100 mg Processing Manual Main sample type Plant samples Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA Format Spin column Technology Silica technology Yield 25–60 µg Time per run or per prep 30 minutes Resources Safety Data Sheets (1) Brochures & Guides (2) Quick-Start Protocols (1) User-Developed Protocols (2) Supplementary Protocols (1) Kit Handbooks (1) Publications Transcriptional profiles of the response to ketoconazole and amphotericin B in Trichophyton rubrum. Yu L; Zhang W; Wang L; Yang J; Liu T; Peng J; Leng W; Chen L; Li R; Jin Q; Antimicrob Agents Chemother; 2006; 51 (1):144-53 2006 Oct 23 PMID:17060531 Glutathione reductase in leaves of cowpea: cloning of two cDNAs, expression and enzymatic activity under progressive drought stress, desiccation and abscisic acid treatment. Contour-Ansel D; Torres-Franklin ML; Cruz DE Carvalho MH; D'Arcy-Lameta A; Zuily-Fodil Y; Ann Bot; 2006; 98 (6):1279-87 2006 Sep 28 PMID:17008354 Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. Kujawa M; Ebner H; Leitner C; Hallberg BM; Prongjit M; Sucharitakul J; Ludwig R; Rudsander U; Peterbauer C; Chaiyen P; Haltrich D; Divne C; J Biol Chem; 2006; 281 (46):35104-15 2006 Sep 19 PMID:16984920 Additional freeze hardiness in wheat acquired by exposure to -3 degreesC is associated with extensive physiological, morphological, and molecular changes. Herman EM; Rotter K; Premakumar R; Elwinger G; Bae H; Ehler-King L; Chen S; Livingston DP 3rd; J Exp Bot; 2006; 57 (14):3601-18 2006 Sep 12 PMID:16968883 Dual effects of plant steroidal alkaloids on Saccharomyces cerevisiae. Simons V; Morrissey JP; Latijnhouwers M; Csukai M; Cleaver A; Yarrow C; Osbourn A; Antimicrob Agents Chemother; 2006; 50 (8):2732-40 2006 Aug PMID:16870766 FAQ Which kit should I use for RNA isolation from Cartilage?
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? Do you have a kit for RNA isolation from any kind of sample type?
What is the composition of Buffer RLT?
Frequently purchased together DNeasy Plant Mini Kit (50) CAT NO./ID 69104 DNeasy Plant Mini Kit (50) 50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml) $305.00 DNeasy Plant Maxi Kit (24) CAT NO./ID 68163 DNeasy Plant Maxi Kit (24) 24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml) $474.00 RNase-Free DNase Set (50) CAT NO./ID 79254 RNase-Free DNase Set (50) 1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps $143.00 DNeasy Plant Mini Kit (250) CAT NO./ID 69106 DNeasy Plant Mini Kit (250) 250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml) $1,344.00 DNeasy Blood & Tissue Kit (50) CAT NO./ID 69504 DNeasy Blood & Tissue Kit (50) 50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml) $215.00 QIAquick PCR Purification Kit (50) CAT NO./ID 28104 QIAquick PCR Purification Kit (50) For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) $141.00 QIAquick Gel Extraction Kit (50) CAT NO./ID 28704 QIAquick Gel Extraction Kit (50) For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) $153.00 QIAprep Spin Miniprep Kit (50) CAT NO./ID 27104 QIAprep Spin Miniprep Kit (50) For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml) $123.00 QuantiTect Rev. Transcription Kit (50) CAT NO./ID 205311 QuantiTect Rev. Transcription Kit (50) For 50 x 20 µl reactions: 100 µl 7x gDNA Wipeout Buffer, 50 µl Quantiscript Reverse Transcriptase, 200 µl 5x Quantiscript RT Buffer, 50 µl RT Primer Mix, 1.9 ml RNase-Free Water $490.00 DNeasy Plant Pro Kit (50) CAT NO./ID 69204 DNeasy Plant Pro Kit (50) Tissue Disruption Tubes, MB Spin Columns, Buffers, Collection Tubes (1.5 and 2 ml), for 50 preps $336.00 DNeasy PowerSoil Pro Kit (50) CAT NO./ID 47014 DNeasy PowerSoil Pro Kit (50) For the isolation of microbial genomic DNA from all soil types. $454.00 RNeasy Mini Kit (50) CAT NO./ID 74104 RNeasy Mini Kit (50) 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers $419.00 Tungsten Carbide Beads, 3 mm (200) CAT NO./ID 69997 Tungsten Carbide Beads, 3 mm (200) 200 tungsten carbide beads (3 mm diameter), suitable for use with TissueLyser systems $291.00 QIAamp Fast DNA Stool Mini Kit (50) CAT NO./ID 51604 QIAamp Fast DNA Stool Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX Buffer, wash and elution buffers, Collection Tubes (2 ml) $359.00 QIAGEN Plasmid Midi Kit (25) CAT NO./ID 12143 QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers $379.00 QIAGEN OneStep RT-PCR Kit (100) CAT NO./ID 210212 QIAGEN OneStep RT-PCR Kit (100) For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml) $759.00 RNeasy PowerPlant Kit (50) CAT NO./ID 13500-50 RNeasy PowerPlant Kit (50) For isolating total RNA from difficult plant and seed samples $398.00 DNeasy mericon Food Kit (50) CAT NO./ID 69514 DNeasy mericon Food Kit (50) 50 QIAquick Spin Columns, Proteinase K, buffers $210.00 Country and Language
From this description, please make a small markdown document describing this product with links to the product and supplier and the most important information about it for a wiki that contains information about this product for scientists to use when they are making purchases for a new lab.
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# RNeasy Plant Mini Kit
The RNeasy Plant Mini Kit by [Qiagen](https://www.qiagen.com) is designed for the efficient purification of high-quality total RNA from a wide array of plant and fungal samples.
## Key Information
- **Supplier:** [Qiagen](https://www.qiagen.com)
- **Cat. No.:** 74904
- **Price:** $507.00 for 50 preps
- **Kit Components:** 50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
## Features
- Yields high-quality total RNA in just 30 minutes.
- Eliminates the need for phenol/chloroform extraction, CsCl gradients, LiCl, or ethanol precipitation.
- Facilitates excellent recovery of RNA and provides RNA ready for any downstream application.
## Applications
Suitable for various downstream applications, including:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
- Poly A+ RNA selection
## Product Details
The kit utilizes QIAshredder spin columns for homogenizing and filtering plant or fungal lysates and RNeasy spin columns for purifying up to 100 µg of RNA using silica-membrane technology. It is compatible with automation on the QIAcube Connect and can be used with TissueRuptor or TissueLyser systems for sample disruption and homogenization.
## Performance
Ideal for isolating total RNA from:
- A wide variety of plant samples (e.g., Arabidopsis thaliana, maize, tomato)
- Filamentous fungi samples
- Sample sizes ranging from 10–100 mg tissue or 100–1x107 cells
The typical yield from 100 mg of plant tissue ranges between 25 µg and 65 µg of RNA.
## Principle
The kit combines guanidine-isothiocyanate lysis with silica-membrane purification technology, ensuring the highest-quality RNA with minimal co-purification of DNA. An on-column DNase treatment can be performed to remove residual DNA, if necessary.
## Procedure
The protocol involves lysing and homogenizing the samples using QIAshredder columns, adding ethanol to the lysate to provide ideal binding conditions, and then purifying the RNA on RNeasy silica membranes. Pure RNA is eluted in water.
[Product Link](https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/rna-purification/total-rna/rneasy-plant-mini-kit)
This product is intended for molecular biology applications and is not for diagnostic use.
For additional information and resources, including safety data sheets, brochures, guides, and kit handbooks, please visit Qiagen's official website.
This markdown document provides concise information about the RNeasy Plant Mini Kit suitable for inclusion in a wiki geared toward helping scientists make informed purchasing decisions for their laboratories.