doi.bio/sriram_subramaniam

Sriram Subramaniam

Career

Sriram Subramaniam is a professor and researcher in the fields of neurology, immunology, biochemistry, and molecular biology.

Subramaniam received his MBBS from the University of Madras, India, and completed his internship in Internal Medicine at Ford Medical Center in Detroit, Michigan. He then undertook residencies at Wayne State University Affiliated Hospitals and Stanford University Medical Center, receiving board certification in Neurology from the American Board of Psychiatry and Neurology in 1983.

Subramaniam served as Acting Assistant Professor of Neurology at Stanford University School of Medicine, before moving to the University of Vermont College of Medicine as an Assistant Professor of Neurology, receiving a promotion to Associate Professor of Neurology.

In 1993, he relocated to Nashville, Tennessee, and established the Multiple Sclerosis Center at Vanderbilt University Medical Center, the first of its kind in the SouthEastern region. During this period, he also held the position of Assistant Professor of Neurology at the University of British Columbia.

Subramaniam's independent research career commenced at the Johns Hopkins School of Medicine, where he progressed from Assistant Professor to Associate Professor. He then transitioned to the National Cancer Institute, NIH, as a Senior Investigator and Chief of the Biophysics Section in the Laboratory of Cell Biology.

He currently holds the position of Professor in the Department of Biochemistry and Molecular Biology and the Department of Urology at the University of British Columbia (UBC). Additionally, he serves as the Gobind Khorana Canada Excellence Research Chair in Precision Cancer Drug Design.

Research Focus

Subramaniam's research focuses on structural biology, drug design, and cryo-electron microscopy (cryo-EM). He has been recognised for his pioneering contributions in the field of cryo-EM, including being the first to demonstrate atomic resolution cryo-EM of proteins and small-molecule drug mapping. His research employs an interdisciplinary approach, combining 3D imaging, computation and machine learning, and cell and molecular biology.

Notable Publications

Subramaniam, S., Kleywegt, G.J. (2022, January 11). A paradigm shift in structural biology. Nature Methods 19(1), 20-23.
Mannar D, Saville JW, Zhu X, Srivastava SS, Berezuk AM, Tuttle KS, Marquez C, Sekirov I, Subramaniam S. (2022, January 20). SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein–ACE2 complex. Science.
Zhu, X, Clarke, R, Puppala, AK, Chittori, S, Merk, A, Merrill B.J, Simonovic, M, Subramaniam, S. (2019). Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9. Nat Struct Mol Biol. 26(8):679-685.
Chittori, S, Hong, J, Saunders, H, Feng, H, Ghirlando, R, Kelly, AE, Bai, Y, Subramaniam, S. (2018). Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N. Science 359(6373), 339-343.

Awards and Recognition

National Cancer Institute Research Highlights Award (2013)
Federal Technology Transfer Award (2015)
NIH Director’s Award for Scientific Excellence (2015)
Orloff Award from NHLBI, NIH for outstanding scientific contribution (2016)
Elected Fellow of the Biophysical Society (2018)
Nomination for the Breakthrough Prize in the Life Sciences

Google Scholar Profile

Sriram Subramaniam)

Google Scholar

Sriram Subramaniam Professor, University of British Columbia N/A Molecular architecture of native HIV-1 gp120 trimers J Liu, A Bartesaghi, MJ Borgnia, G Sapiro, S Subramaniam Nature 455 (7209), 109, 2008 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:ufrVoPGSRksC Cited by: 1020

Breaking cryo-EM resolution barriers to facilitate drug discovery A Merk, A Bartesaghi, S Banerjee, V Falconieri, P Rao, MI Davis, … Cell 165 (7), 1698-1707, 2016 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:YohjEiUPhakC Cited by: 596

2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor A Bartesaghi, A Merk, S Banerjee, D Matthies, X Wu, JLS Milne, … Science 348 (6239), 1147-1151, 2015 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:Ri6SYOTghG4C Cited by: 575

Molecular mechanism of vectorial proton translocation by bacteriorhodopsin S Subramaniam, R Henderson Nature 406 (6796), 653, 2000 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:buQ7SEKw-1sC Cited by: 567

Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. S Subramaniam, M Gerstein, D Oesterhelt, R Henderson The EMBO journal 12 (1), 1-8, 1993 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:SP6oXDckpogC Cited by: 461

Mitochondrial reticulum for cellular energy distribution in muscle B Glancy, LM Hartnell, D Malide, ZX Yu, CA Combs, PS Connelly, … Nature 523 (7562), 617, 2015 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:fbc8zXXH2BUC Cited by: 425

Site-specific 3D imaging of cells and tissues with a dual beam microscope JAW Heymann, M Hayles, I Gestmann, LA Giannuzzi, B Lich, … Journal of structural biology 155 (1), 63-73, 2006 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:vRqMK49ujn8C Cited by: 398

2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition S Banerjee, A Bartesaghi, A Merk, P Rao, SL Bulfer, Y Yan, N Green, … Science 351 (6275), 871-875, 2016 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:OR75R8vi5nAC Cited by: 372

Cryo‐electron microscopy–a primer for the non‐microscopist JLS Milne, MJ Borgnia, A Bartesaghi, EEH Tran, LA Earl, DM Schauder, … The FEBS journal 280 (1), 28-45, 2013 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:Ug5p-4gJ2f0C Cited by: 364

Protein conformational changes in the bacteriorhodopsin photocycle S Subramaniam, M Lindahl, P Bullough, AR Faruqi, J Tittor, D Oesterhelt, … Journal of molecular biology 287 (1), 145-161, 1999 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:8d8msizDQcsC Cited by: 290

Structural mechanism of trimeric HIV-1 envelope glycoprotein activation EEH Tran, MJ Borgnia, O Kuybeda, DM Schauder, A Bartesaghi, … PLoS pathogens 8 (7), e1002797, 2012 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:dTyEYWd-f8wC Cited by: 273

Three-dimensional structure of a bacterial oxalate transporter T Hirai, JAW Heymann, D Shi, R Sarker, PC Maloney, S Subramaniam Nature Structural & Molecular Biology 9 (8), 597, 2002 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:maZDTaKrznsC Cited by: 268

Cryo-EM structure of human rhodopsin bound to an inhibitory G protein (vol 558, pg 553, 2018) Y Kang, O Kuybeda, PW de Waal, S Mukherjee, N Van Eps, P Dutka, … NATURE 561 (7724), E44-E44, 2018 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:lgwcVrK6X84C Cited by: 259

Structural mechanism of glutamate receptor activation and desensitization JR Meyerson, J Kumar, S Chittori, P Rao, J Pierson, A Bartesaghi, … Nature 514 (7522), 328, 2014 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:9c2xU6iGI7YC Cited by: 256

Cryo-EM structure of human rhodopsin bound to an inhibitory G protein Y Kang, O Kuybeda, PW de Waal, S Mukherjee, N Van Eps, P Dutka, … Nature 558 (7711), 553, 2018 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:PaBasH6fAo0C Cited by: 255

3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells RL Felts, K Narayan, JD Estes, D Shi, CM Trubey, J Fu, LM Hartnell, … Proceedings of the National Academy of Sciences 107 (30), 13336-13341, 2010 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:nVrZBo8bIpAC Cited by: 231

Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures A Harris, MJ Borgnia, D Shi, A Bartesaghi, H He, R Pejchal, YK Kang, … Proceedings of the National Academy of Sciences 108 (28), 11440-11445, 2011 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:gVv57TyPmFsC Cited by: 223

Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entry R Sougrat, A Bartesaghi, JD Lifson, AE Bennett, JW Bess, DJ Zabransky, … PLoS pathogens 3 (5), e63, 2007 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:yFnVuubrUp4C Cited by: 223

Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy P Zhang, CM Khursigara, LM Hartnell, S Subramaniam Proceedings of the National Academy of Sciences 104 (10), 3777-3781, 2007 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:738O_yMBCRsC Cited by: 223

Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy A Bartesaghi, D Matthies, S Banerjee, A Merk, S Subramaniam Proceedings of the National Academy of Sciences 111 (32), 11709-11714, 2014 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:ye4kPcJQO24C Cited by: 221

Google Scholar

Sriram Subramaniam

Professor, University of British Columbia

N/A

Molecular architecture of native HIV-1 gp120 trimers J Liu, A Bartesaghi, MJ Borgnia, G Sapiro, S Subramaniam Nature 455 (7209), 109, 2008 Link: https://scholar.google.com/citations?viewop=viewcitation&hl=en&user=YQhbBX8AAAAJ&citationforview=YQhbBX8AAAAJ:ufrVoPGSRksC