doi.bio/qiagen/rneasy_plant_mini_kit

RNeasy Plant Mini Kit (50)

Qiagen Cat. No. / ID: 74904

50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

$507.00 Quantity 1

RNeasy Plant Mini Kit

The RNeasy Plant Mini Kit by Qiagen is designed for the efficient purification of high-quality total RNA from a wide array of plant and fungal samples.

Key Information

• Supplier: Qiagen
• Cat. No.: 74904
• Price: $507.00 for 50 preps
• Kit Components: 50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

Features

• Yields high-quality total RNA in just 30 minutes.
• Eliminates the need for phenol/chloroform extraction, CsCl gradients, LiCl, or ethanol precipitation.
• Facilitates excellent recovery of RNA and provides RNA ready for any downstream application.

Applications

Suitable for various downstream applications, including:

• Northern, dot, and slot blotting
• End-point RT-PCR
• Quantitative, real-time RT-PCR
• Array analysis
• Poly A+ RNA selection

Product Details

The kit utilizes QIAshredder spin columns for homogenizing and filtering plant or fungal lysates and RNeasy spin columns for purifying up to 100 µg of RNA using silica-membrane technology. It is compatible with automation on the QIAcube Connect and can be used with TissueRuptor or TissueLyser systems for sample disruption and homogenization.

Performance

Ideal for isolating total RNA from:

• A wide variety of plant samples (e.g., Arabidopsis thaliana, maize, tomato)
• Filamentous fungi samples
• Sample sizes ranging from 10–100 mg tissue or 100–1x107 cells

The typical yield from 100 mg of plant tissue ranges between 25 µg and 65 µg of RNA.

Principle

The kit combines guanidine-isothiocyanate lysis with silica-membrane purification technology, ensuring the highest-quality RNA with minimal co-purification of DNA. An on-column DNase treatment can be performed to remove residual DNA, if necessary.

Procedure

The protocol involves lysing and homogenizing the samples using QIAshredder columns, adding ethanol to the lysate to provide ideal binding conditions, and then purifying the RNA on RNeasy silica membranes. Pure RNA is eluted in water.

Product Link

This product is intended for molecular biology applications and is not for diagnostic use.

For additional information and resources, including safety data sheets, brochures, guides, and kit handbooks, please visit Qiagen's official website.

1. Thermo Fisher Scientific - Invitrogen™ Plant RNA Purification Kit

• Website: Thermo Fisher Scientific
• Specifically designed for high-purity RNA extraction from a wide range of plant species. Utilizes a column-based purification process similar to the RNeasy kit.

1. Promega - Maxwell® RSC Plant RNA Kit

• Website: Promega
• This kit is designed for automated RNA purification from plant tissue using the Maxwell® RSC Instrument, providing an automated solution similar to the QIAcube Connect automation of the RNeasy kit.

1. Sigma-Aldrich - Spectrum™ Plant Total RNA Kit

• Website: Sigma-Aldrich
• Offers a method for extraction and purification of total RNA from plant tissues, emphasizing a protocol that reduces the co-purification of polysaccharides and phenolic compounds.

1. Bio-Rad - Aurum™ Total RNA Mini Kit

• Website: Bio-Rad
• Suitable for extracting high-quality total RNA from plant cells, tissues, and fungi, providing a silica membrane-based column purification method.

1. Agilent Technologies - Plant RNA Isolation Mini Kit

• Website: Agilent Technologies
• Designed to isolate and purify total RNA from a wide variety of plant materials, using a spin column-based method to remove contaminants and inhibitors.

1. Zymo Research - Direct-zol™ RNA Miniprep Plus

• Website: Zymo Research
• Capable of processing plant tissue directly without the need for separate DNA removal steps. It is suitable for a broad range of plant types and sample sources.

Each of these kits offers a proprietary approach to RNA isolation and purification, with unique features tailored to specific types of samples or user needs, such as automation compatibility, specific types of contaminants addressed, or the range of sample types that can be processed. When choosing an RNA extraction kit, scientists should consider the specific requirements of their experiments, such as the expected yield, purity needed for downstream applications, the throughput of samples, and the types of samples being processed.

qiagen/rneasyplantminikitorig